FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34773276
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT968970.2786536419519403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG184000.027.9559215
AGACCGC184800.027.895784
ACCGCGT189900.027.1818036
CAGACCG190600.027.117743
CCGCGTT206900.024.8286297
CGCGTTC214500.023.957798
TCAGACC226450.023.5004122
GAACGAT236500.021.49060645
GCGTTCT249900.020.6081689
CTCAGAC283550.020.4129641
AGAACGA263200.019.42175744
ATACGGA262250.019.2008633
AATACGG262650.019.17175532
TACGGAG264600.018.81769434
CAATACG280200.018.18789331
AAGAACG282900.018.08539243
ACGGAGA282050.017.67740235
CGTTCTC300550.017.57688110
CCCAATA297700.017.50429229
CGGAGAG295200.016.81358136
Produced by FastQC (version 0.11.5)