FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26301372
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1341700.5101254793856381No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA789290.3000946110339795No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG542280.20617935824792716No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG415170.15785108092459968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC268150.025.658354
CAGACCG273950.025.2301583
GACCGCG283900.024.2982735
TCAGACC297750.023.3969142
ACCGCGT296300.023.2889966
CTCAGAC335050.021.8667131
TTCGGCG68050.018.74748444
CCCGCGT14300.018.5675541
TCGGCGC71750.017.62254345
CCGCGTT399000.017.3058347
CGCGTTC432050.015.9768048
CTACACG24950.015.51001145
CCGTACG82850.015.5013884
CGTACGC83800.015.2182975
GCGTTCT464750.014.8671889
TCCGTAC88750.014.622993
TACGCCA89650.014.3506887
CGCGTAA11050.014.2542531
GTACGCC91700.014.0298746
CCCGTAT16100.013.8362381
Produced by FastQC (version 0.11.5)