FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43831807
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1080550.2465218921957746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29200.025.0320766
GACCGCG82400.021.9172065
AGACCGC86900.020.834874
GTTCGAC34600.020.605355
ACCGCGT88000.020.4968936
CGGTATA47900.020.09647820
CGACGAG35800.019.7892048
TCGACGA38100.019.3618437
CAGACCG97850.018.664033
GCACTAC81550.018.1758625
CCGTACG31250.017.129384
CGGCAGT346600.017.08737440
CGTACGC32250.016.5975535
CCGCGTT109350.016.4950437
GTCCGTT29750.016.1157971
ATGACGG58300.015.54719916
GACGGTA62100.015.53734918
GACGGCA382450.015.52813338
TGACGGC392950.015.14771937
AGTTGCA392950.015.09219544
Produced by FastQC (version 0.11.5)