FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43831807
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG665940.15193076571084554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG22900.015.32597326
AGGGATC115950.011.0232382
TAATCGG35650.010.7316894
TGTCGTT33050.010.550428
GTAACGA34600.010.46996518
ATTTCTA380800.010.3826072
TCTATAG373100.010.3687015
CATTTCT408650.010.1112491
GCTAAGT37800.010.0600414
TTTCTAT395400.010.0561663
TTCTATA394800.09.8958084
ATCGGTT43050.09.6710976
CTATAGT410750.09.5389026
AATCGGT40050.09.4963765
GTCGTTC38600.09.3829729
TATAGCT42350.09.2995267
ATATAGC45100.09.2814036
TATAGTG421850.09.2772177
AACTCTA97400.09.19314345
ATAATCG42700.09.1696763
Produced by FastQC (version 0.11.5)