FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38206927
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1580050.41355066320827116No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG395750.1035806936265772No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA387670.10146589386788422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC332950.023.5020394
GACCGCG333950.023.4181945
ACCGCGT343650.022.7570926
CAGACCG348150.022.5604743
TCAGACC381700.020.8958642
CCGCGTT377700.020.6995667
CGCGTTC385750.020.2792598
CTCAGAC420500.019.6495531
GAACGAT404400.019.2239845
GCGTTCT426700.018.3910629
AGAACGA462700.016.8798444
CGTTCTC486850.016.3406110
AATACGG500200.015.52975532
AAGAACG501650.015.5235543
ATACGGA500650.015.52137333
TACGGAG503100.015.42889234
TTCGACG15950.015.3707216
CAATACG511550.015.25744931
CTACACG37600.014.956645
CCTCTCA533100.014.92502419
Produced by FastQC (version 0.11.5)