FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34098688
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC50600.023.2514554
GACCGCG55550.021.3010315
ACCGCGT59450.019.8279766
CAGACCG61150.019.6446253
TTCGACG14050.019.0532746
CCGTACG40450.016.405994
CGTACGC41000.016.185915
CCGCGTT77500.015.355027
TCGACGA19500.014.4202177
TACGCCA48450.014.1612797
CTCGGTT60000.013.9216211
CGGTATA28250.013.85677620
CGCGTAA10150.013.7528551
TCCGTAC49500.013.7246313
TCAGACC96950.013.4347922
CCCGTAT20500.013.3990241
CTCGTAT39650.013.3441781
CGCGTTC90150.013.20037658
CTCGATT39850.013.0512111
GTCCGTT23200.012.7130721
Produced by FastQC (version 0.11.5)