FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47125475
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT478130.1014589242867048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG118700.020.8872035
AGACCGC119550.020.6828964
ACCGCGT125050.019.8815446
CAGACCG134100.018.7908133
CCGCGTT141450.017.5923637
CGCGTTC146250.016.9995868
TTCGACG19850.014.7351456
TCAGACC177150.014.73238852
AACGCTA119250.013.82841523
ACGCTAC120800.013.66993824
CTCAGAC219900.013.5303131
CGCTACC127400.012.90878525
TCGACGA23800.012.8568317
GCGTTCT200150.012.6352189
GTCCGTT32850.012.1130761
AAAACGC139750.011.99292921
GAACGAT209700.011.897325545
AATACGG216050.011.64164532
TACGGAG216000.011.51907434
ATACGGA219600.011.49414733
Produced by FastQC (version 0.11.5)