FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43486944
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1016440.23373452041145956No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG737420.16957273429008946No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA520140.11960831278463717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC216150.026.4591434
GACCGCG215450.026.2109455
CAGACCG222500.025.8455813
ACCGCGT221950.025.4331996
TCAGACC260250.023.5143852
CTCAGAC294500.022.3475111
TTCGACG20350.020.011046
CCGCGTT298600.018.9346877
CGCGTTC331350.017.0632218
ACGTCCG18600.017.0553932
TCGACGA24700.016.6690037
CGGTATA32800.015.50284920
GCGTTCT378550.014.98916159
CGTTCTC439700.013.64140610
GTTTTCG343700.013.49082345
CGACGAG29250.013.46078
ACGCTAC355100.013.39449224
AACGCTA355150.013.34204723
GTCGCTA23050.013.07944527
CGCTACC365550.012.95569625
Produced by FastQC (version 0.11.5)