FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32767348
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1335840.4076741273050233No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1204540.3676037499281297No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG603630.18421692228495268No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA588450.1795842617473956No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC583400.17804309338674584No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT558930.17057529342930042No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG481490.14694201068698023No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA444150.13554652027377986No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG433130.13218341624717386No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA428000.1306178333382366No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA409070.12484074084970197No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG338680.10335899017521956No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT329120.1004414516548608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC206500.015.4994052
AGCTTAG146950.014.45984845
GTCACTC195850.014.1660988
CAGGGAT235750.014.1295891
ACCCGGC59000.013.7344265
GGTCACT201650.013.5246627
ACTCTCC205050.013.49816311
GGCATAG159250.013.49074335
ATAGCTC56550.013.411386539
GGGAGGT216500.013.3495453
ATAGGGG160750.013.29937238
AGGTCAC213200.013.1401866
GAGGTCA220900.012.8798675
GGAGGTC218000.012.8447964
CATTTCT570800.012.7479771
GAGCTTA168600.012.68309944
TCACTCT227250.012.4464299
GGGATCA257750.012.3221843
CACTCTC223450.012.27546810
TAGCTCT62300.012.24724840
Produced by FastQC (version 0.11.5)