FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41400490
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1547570.3738047544847899No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1449740.3501745993827609No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT829410.20033820855743495No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG828920.20021985247034516No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA461720.11152525006346543No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG415910.1004601636357444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC197450.023.5264234
CAGACCG205800.022.8675463
GACCGCG227450.020.2840775
TCAGACC244000.020.2485642
CTCAGAC278200.018.9301171
CCCGCGT21000.018.7869531
ACCGCGT252650.018.3313666
CCGCGTT454400.018.1287481
CGCGTTC504650.016.2813682
CGGCAGT557350.015.59080840
TTCGACG13600.015.5470056
AGTTGCA569200.015.2113844
GCGTTCT557200.015.1980443
CCGTACG72550.014.9359224
CGTACGC72900.014.9255695
AACGCTA179900.014.36665223
GCACTAC62050.014.35561125
CGACCGC17450.014.3405281
ACGGCAG609450.014.30275539
CGTTCTC607800.014.20700554
Produced by FastQC (version 0.11.5)