FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41400490
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1198270.2894337724022107No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG873760.21105064215423538No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA870910.21036224450483557No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG746480.18030704467507508No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT709180.1712974894741584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG356400.018.19270145
GGGAGGT508000.017.9349823
GGAGGTC517650.017.2212924
GAGGTCA519200.017.1172085
TGGGAGG401700.017.1158982
GGTCACT528950.016.3555187
CTGGGAG448800.016.2451231
GGCATAG406350.016.09500535
ATAGGGG408650.016.0545338
GTCACTC535350.016.0507768
GAGCTTA418850.015.79970644
TGGCATA423250.015.52676434
ACTCTCC571150.015.09941811
TCACTCT575250.015.0314389
CACTCTC572450.014.86121510
CACCCGG115650.014.5092121
AGGGATC184600.014.2321882
AGGTCAC628450.014.0380956
GTTGTCG22900.013.94812326
AGGCTCT502600.013.65055320
Produced by FastQC (version 0.11.5)