FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32004053
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1099940.3436877198022388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC204450.026.7858854
GACCGCG210500.026.0053425
ACCGCGT214500.025.5518636
CAGACCG217400.025.3044343
CCGCGTT236200.023.156687
TCAGACC246250.022.9247042
CGCGTTC246250.022.1933298
CTCAGAC283600.021.1623551
GCGTTCT275750.019.884289
CGTTCTC328450.017.21444710
TTCGGCG45850.017.12644
GAACGAT327850.016.44530345
CCGTACG51350.015.6422894
TCGGCGC51000.015.48699745
AGAACGA354900.015.35458244
CGTACGC52450.015.228445
TACGGAG361200.014.974668534
ATACGGA364650.014.9255933
AATACGG365950.014.88486532
CCTCTCA376700.014.84885619
Produced by FastQC (version 0.11.5)