FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39409435
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2708170.6871882329700997No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA514760.13061846737970234No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA401620.10190960616410766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC457700.028.8212874
GACCGCG462500.028.5352445
CAGACCG464050.028.4947683
ACCGCGT471500.028.0144146
TCAGACC485500.027.4953142
CTCAGAC537400.025.6448381
CCGCGTT533050.024.7793757
CGCGTTC559250.023.6104568
GAACGAT559450.022.9959945
GCGTTCT600950.022.0694459
AGAACGA610200.021.06509844
AAGAACG633900.020.26317443
ATACGGA636500.020.22343433
AATACGG637500.020.1971232
TACGGAG642200.020.03453834
CGTTCTC668700.019.95119310
CAATACG653800.019.78624531
TTCGACG17750.019.5163946
ACGGAGA665200.019.37493135
CCCAATA678450.019.0857429
Produced by FastQC (version 0.11.5)