FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39409435
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA672990.17076875118864301No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG450230.11424421588383593No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG416210.1056117653044252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG126350.014.86295138
GGCATAG128550.014.46127135
AGCTTAG129350.014.36828245
ATATAGC38550.014.1645546
GTTGTCG34000.014.02224826
GTCACTC227100.013.93438158
GGTCACT227800.013.8002767
CGTAGGG95350.012.9175737
GGCGTAG95650.012.89419135
GGGAGGT260200.012.872053
ACTCTCC244100.012.83938211
AGGTCAC251400.012.7817936
TGTCGTT36900.012.67678928
GCGTAGG97750.012.54980136
GAGCTTA151750.012.51882844
CATAGGG151050.012.50706737
TATAGCT44800.012.4895017
GGAGGTC262550.012.481214
TAGGGGA254950.012.12632639
TTGTCGT42850.011.96632527
Produced by FastQC (version 0.11.5)