FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41956067
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG122600.014.38576345
ATAGGGG128350.013.88490538
GGCATAG132400.013.27478335
GTTGTCG20400.012.90408726
GAGCTTA146900.012.09830644
CATAGGG149250.011.89600637
TGGCATA148650.011.85390134
GGGAGGT217850.011.7017883
GCATAGG159000.011.26577836
GGAGGTC219400.011.116844
CGTGGCG83950.010.8009041
ACTCTCC226200.010.29501811
TAGGGGA245650.09.68147939
CGACCCG92750.09.65477856
GGCGACC93300.09.5740914
TGGCGAC94700.09.5036383
TCTCCCC249650.09.30971113
GTGGCGA95800.09.2770612
TCTATAG217000.09.2695293
GCGACCC98550.09.1784755
Produced by FastQC (version 0.11.5)