FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33897306
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA579980.17109914280503588No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG482690.14239774688879406No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG355280.10481068908543942No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG349040.10296983482994193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG133750.013.96313445
ATAGGGG139150.013.72726338
GGCATAG144500.013.0625735
GGGAGGT204350.012.9429453
TGGCATA149600.012.72237434
GAGCTTA152250.012.41438144
GTCACTC199850.012.1484738
GGAGGTC208250.012.1171384
GGTCACT199450.012.0598247
CATAGGG165250.011.64033737
ACTCTCC214150.011.41100311
AGGGATC125700.010.9771412
GCATAGG179550.010.68818336
TCTCCCC239850.010.17918513
TCAGACG77650.010.17489052
CACTCTC242150.09.99860110
CGTGGCG118750.09.9704831
GTGGCGA118750.09.9136172
GTTGTCG15000.09.8983526
TGGGAGG212350.09.88993552
Produced by FastQC (version 0.11.5)