FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38008188
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT473260.12451527549800585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC121050.021.0893384
GACCGCG123700.020.8011655
ACCGCGT128850.019.9871676
CAGACCG135800.019.0968363
CCGCGTT150300.017.0898117
CTACACG50000.016.870945
CGCGTTC154300.016.5593228
TTCGACG15050.016.2899656
TCAGACC165250.015.8432592
ACGCTAC133500.015.49811224
AACGCTA136200.015.32305923
CGCTACC141400.014.47318725
CTCAGAC206150.014.0607881
GTCCGTT30900.013.63005351
AAAACGC155750.013.44140221
CGGTATA24150.013.42018220
GCGTTCT195550.013.1812619
AAACGCT165800.012.66826322
GAAAACG167550.012.61345920
TCGACGA20100.012.0853117
Produced by FastQC (version 0.11.5)