FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21376194
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT425890.1992356543919839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG94400.026.4614665
ACCGCGT98350.025.7127916
AGACCGC98850.025.680084
CAGACCG103150.024.6972313
CCGCGTT104350.024.212217
CGCGTTC106850.023.6454878
TCAGACC126700.021.7764952
GAACGAT124550.020.81048245
GCGTTCT124500.020.4017129
CTCAGAC151850.019.7859941
TACGGAG129100.019.186634
ATACGGA134200.019.09458433
AATACGG135050.018.99119432
CAATACG145150.018.13514331
AGAACGA143700.018.08269744
ACGGAGA144150.017.32388535
AAGAACG152400.017.1389143
CGTTCTC161800.016.96380810
CGGAGAG147100.016.62466236
CCCAATA166900.016.4599229
Produced by FastQC (version 0.11.5)