FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39417764
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG682910.17324929947827583No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG540770.1371894154117925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27800.025.169946
CGGTATA43450.021.85337320
CGACGAG32600.021.3236058
TCGACGA32800.021.1941497
GTTCGAC36000.019.374295
AGACCGC96550.018.8774054
GACCGCG99300.018.466095
CAGACCG104650.017.86793
AACGCTA250500.017.48192823
ACGCTAC250300.017.44201924
ACCGCGT107350.017.1023086
CGCTACC255700.017.0824925
AAAACGC269650.016.33956721
AAACGCT279350.015.76456722
GAAAACG281450.015.73329320
CGTGGTT288750.015.2708141
CCGGCTT291750.015.2015392
GCCGGCT249700.015.1135521
AGTTCGA48750.014.9547784
GTTTTCG296800.014.77302345
Produced by FastQC (version 0.11.5)