FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32041833
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1215020.37919803152335263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG223550.028.8057945
AGACCGC225250.028.7881724
ACCGCGT229400.028.139876
CAGACCG238850.027.4127543
CCGCGTT245950.025.9982747
TCAGACC264250.025.578792
CGCGTTC252900.025.2304738
CTCAGAC311400.023.5135651
GAACGAT284500.022.56638545
GCGTTCT283800.022.5467789
AATACGG304500.020.9476432
AGAACGA307300.020.88846444
TACGGAG305200.020.82587234
ATACGGA308600.020.70578833
CAATACG322450.019.96298431
ACGGAGA324650.019.62660435
CGTTCTC335050.019.59489410
AAGAACG335000.019.18810343
CCCAATA346400.018.84299929
CCCCAAT351650.018.6000728
Produced by FastQC (version 0.11.5)