FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32041833
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC81500.015.345
ATAGCTC74450.015.2011339
TCACCCG85900.014.4237943
ACGGACA85000.014.21746618
TAGCTCT83450.013.61322440
CGGACAC93050.013.27139713
CCGGACA93100.013.11838412
CACCCGG95450.013.0745264
CGGACAG91700.013.03248819
TTGATAG92350.012.94209236
TGATAGC93750.012.72388137
GATAGCT93050.012.407338
GGATTGA103900.011.80915325
TCAGACG60500.011.4850882
ACACGGA103700.011.47705616
AGCTTAG72750.011.46710745
GCCCGGA110950.011.41189410
GGCATAG72300.011.3332435
CACGGAC108550.011.19398317
CGGCCCG119900.010.9348078
Produced by FastQC (version 0.11.5)