FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39776262
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG679920.1709361226552661No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA523030.13149299951815482No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT500810.12590675312828542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC118800.023.578874
CAGACCG130650.021.8557383
GACCGCG130250.021.2808175
ACCGCGT137350.020.1810596
TCAGACC164550.017.7633362
CCCGCGT16000.015.9070081
CTCAGAC220550.015.1448211
CCCGTAT25100.014.7163521
TTCGACG12300.014.6334516
AACGCTA101500.014.32072323
ACGCTAC101550.014.2698624
CCGCGTT210000.013.8678541
CGCTACC108550.013.34981525
GTCCGTT31100.013.3256221
TGACGGC293650.013.04076137
CGGCAGT300700.012.92908740
CCGTACG60400.012.7769534
AGTTGCA293300.012.72526344
ACGGCAG305900.012.628448539
GACGGCA303800.012.58272438
Produced by FastQC (version 0.11.5)