FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32874160
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG469200.14272608030136738No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA435190.13238056881149207No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG378190.11504172273907531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG128650.015.14662445
ATAGGGG139350.014.03872738
GGCATAG143500.013.64652435
GGTCACT192000.013.509117
GTCACTC193500.013.2890438
GGGAGGT207000.013.007213
GAGCTTA152500.012.87171644
TGGCATA158300.012.4641634
CATAGGG162150.012.17515337
GCATAGG167800.011.76493436
ACTCTCC226400.011.59958911
AGGGATC116350.011.5602012
TCACTCT234850.011.3714139
GGAGGTC240000.011.1636414
GAGGTCA255700.010.8911265
TGGGAGG202600.010.4467392
AGGTCAC268800.010.2754546
CAGGGAT146500.010.1642831
TGTCCAA256950.010.13498526
GTTGTCG15100.010.12733426
Produced by FastQC (version 0.11.5)