FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26684866
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT399980.1498902036832413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC113450.022.9724484
CAGACCG118550.022.117573
GACCGCG116700.021.9453475
ACCGCGT117500.021.9304756
CCGCGTT137550.018.7173567
TCAGACC153550.018.6551692
CGCGTTC142850.017.975488
CTCAGAC178100.017.4566381
GCGTTCT163550.015.7970099
CCGTACG23350.014.121214
CGTACGC24150.013.7467625
CGTTCTC207850.013.42432210
GTCCGTT13500.012.9980231
CTCCGAT27150.012.6776291
TTCGACG10700.011.8196466
CTACACG35250.011.60931245
GTCCGGT17250.011.606931
TACGCCA32150.011.30954557
GTACGCC30050.011.2731956
GTCCGCT27200.011.2482881
Produced by FastQC (version 0.11.5)