FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26684866
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA287150.10760781035962481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA184350.010.8792062
ATAGGGG57450.010.61566438
ATAGTGT201000.09.8997146
AGCTTAG60750.09.69684545
TATAGTG205900.09.6204415
CTATAGT198700.09.618264
AGCGCTC39400.09.5878829
GGCATAG60550.09.44366635
TTACTAG202700.09.43278814
TTTCTAT197300.09.3903221
TACTAGA206050.09.30209915
CGGGACG31350.09.18703117
TAGTGTG216800.09.1056627
ATAACGC31150.09.101274539
GGGAGGT106050.09.0954073
CGACCCG45950.09.0034266
TGTGTTA222250.08.95402910
AAGCCGG144850.08.87877445
ACTAGAG215300.08.87175216
GTTACTA218500.08.83214113
Produced by FastQC (version 0.11.5)