FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41348479
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1984670.47998621666349567No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG527740.12763226429683183No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA483510.11693537747785113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC434700.023.7939644
CAGACCG442450.023.4540183
GACCGCG444600.023.2891855
ACCGCGT452450.022.9048126
GAACGAT441950.022.69633545
CCGCGTT477250.021.6957247
TCAGACC488150.021.4479242
CGCGTTC484250.021.2752638
CTCAGAC518600.021.0765931
GCGTTCT510200.020.2372429
AGAACGA510800.019.670244
CGTTCTC559950.018.57580610
ATACGGA576050.017.54741333
AATACGG578050.017.50578332
TACGGAG576700.017.50127434
AAGAACG581600.017.29555543
CAATACG591950.017.0432831
ACGGAGA599750.016.88791835
CCCAATA602950.016.87596129
CACTCCC611100.016.74138824
Produced by FastQC (version 0.11.5)