FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40618578
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1337440.3292680506934536No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1287550.3169854936822259No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA499780.1230422197448665No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG422530.10402382870222586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC261900.026.0864684
CAGACCG267450.025.6470853
GACCGCG267750.025.500455
ACCGCGT276950.024.6812616
TCAGACC305850.022.640472
TTCGGCG73100.021.35328344
CTCAGAC351150.021.0578171
CCGCGTT338650.020.1778417
TCGGCGC81900.019.41587445
CGCGTTC356950.019.1118878
CGGCAGT367800.018.01518440
CCGTACG91950.017.553334
GCGTTCT396450.017.2474239
CGTACGC93600.017.2203565
ACGGCAG397000.016.6901439
GACGGCA403000.016.51420238
GTTCCTA76200.016.4406315
AGTTGCA416450.016.09984244
TACGCCA101850.015.9667047
TTCGACG13600.015.8770696
Produced by FastQC (version 0.11.5)