FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46549320
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2861840.6147973804987914No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA581060.12482674290408538No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG551300.11843352384094978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35500.027.052976
AGACCGC573150.026.413244
CAGACCG580750.026.18753
GACCGCG577250.026.0269645
ACCGCGT584050.025.7627166
TCAGACC627700.024.7298182
TCGACGA39850.024.1567257
CCGCGTT623500.024.0454757
CGGTATA53750.024.0349220
CTCAGAC683700.023.5507491
CGCGTTC637000.023.5465458
GCGTTCT674600.022.2374949
GAACGAT684850.021.59982945
CGACGAG45050.021.2175458
CGTTCTC745700.020.55158410
GTTCGAC50500.019.107145
AGAACGA780650.019.0137744
AATACGG821900.018.26740332
ATACGGA820100.018.26618433
TACGGAG817750.018.19295134
Produced by FastQC (version 0.11.5)