FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46549320
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG906280.1946924251525049No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA827240.17771258527514472No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG788650.16942245343218762No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG578360.1242467129487606No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT500880.10760200149003252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG270350.017.20441245
GTCACTC357050.016.3547638
GGCATAG295200.015.75456135
ATAGGGG298350.015.73255138
GGGAGGT397600.015.7299363
GGTCACT377850.015.5906077
AGGTCAC392400.015.2912616
GGAGGTC401450.015.1991364
GAGCTTA313650.014.92285844
GAGGTCA410450.014.8526885
TGGCATA319000.014.72659834
ACTCTCC399150.014.69350111
TCACTCT411100.014.3531559
CACTCTC420700.013.78711910
CATAGGG340900.013.76846437
TGGGAGG366700.013.3929922
GCATAGG355950.013.1673536
CTGGGAG430900.012.4971781
TCTCCCC484250.012.15641713
AGGGATC166550.011.9326912
Produced by FastQC (version 0.11.5)