FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46806704
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT665130.142101439144273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG153050.024.2592545
ACCGCGT157100.023.734126
AGACCGC159050.023.400694
CAGACCG169600.022.2900563
CCGCGTT181300.020.4665897
TTCGACG18950.019.3555376
CGCGTTC191800.019.3454958
TCAGACC214400.018.3672752
CTCAGAC254300.017.308041
GCGTTCT237250.015.6662249
TCGACGA27200.014.3119847
GTTCCTA93450.013.74736915
GAACGAT252950.012.95057545
CGGTATA33750.012.93329920
CGACGAG27850.012.684768
AATACGG289100.012.54632
ATACGGA290700.012.4690633
CGTTCTC308800.012.45888910
TACGGAG286450.012.44972934
AGTTACC112650.011.84412534
Produced by FastQC (version 0.11.5)