FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45407459
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1809740.39855566461008096No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA797440.1756187237872086No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG589600.12984650825759705No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG532770.11733094335888736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC103900.021.8432524
TTCGACG25500.020.904046
GACCGCG114850.019.643175
CAGACCG117300.019.6355743
CCGCGTT235100.018.0057931
CGGCAGT511650.017.49149140
ACCGCGT130850.017.2232886
TCGACGA32450.016.981647
CGGTATA40800.016.97959920
AGTTGCA540350.016.62254344
CCCGCGT19550.016.3462311
ACGGCAG556350.016.11957739
GACGGCA557550.016.04622338
TGACGGC558450.016.01632937
CGCGTTC265200.015.9453172
CAGTTGC567350.015.907166543
GTCCGTT36650.015.8424291
CGACGAG32450.015.8034278
GTTGCAG572000.015.70410445
TCAGACC154850.015.5279942
Produced by FastQC (version 0.11.5)