FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34950377
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA630240.1803242351291375No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC542970.15535454739157747No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG419670.12007595797893682No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA404970.11586999476429109No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG394760.11294871010976507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20150.020.08878526
ATATAGC29350.016.6150196
ATAGGGG123400.016.4035438
GGCATAG126300.016.05514535
AGCTTAG128800.015.86261945
TGGCATA134950.015.09052434
TATAGCT31600.014.7920347
GAGCTTA137650.014.78161344
TGTCGTT27400.014.77381128
GGGAGGT212900.014.55619053
GGAGGTC217550.014.0369834
AGGGATC109500.013.7387482
GTCGTTC29350.013.71745229
CATAGGG149500.013.69036837
GCTAAGT31050.013.3955114
TTGTCGT33250.013.25676327
TCGTTCC32000.012.86356730
GCGTTCG23250.012.76442715
AGTTAGG30650.012.73004154
TAGGGGA238600.012.29607239
Produced by FastQC (version 0.11.5)