FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44396955
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA737910.16620734462532397No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG615630.1386649151951975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168400.014.75964545
ATAGGGG175600.014.14471738
GGCATAG183400.013.22286435
GAGCTTA188050.013.193583544
GGGAGGT248850.012.7785063
GGAGGTC256200.012.1832784
TGGCATA205850.011.84565534
CATAGGG216500.011.42061437
GTCACTC266650.011.3738968
GGTCACT262400.011.344917
GCATAGG224200.011.0183136
CACCGAA356600.011.00702245
GAGGTCA289000.010.9638015
ACTCTCC278700.010.9213611
AGGTCAC292800.010.71419056
TCACTCT286000.010.6659199
GTTGTCG23650.010.55832526
CACTCTC282600.010.45960110
GCACCGA384000.010.20416644
TGGGAGG267700.09.8260512
Produced by FastQC (version 0.11.5)