FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49097953
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4498300.916188909138432No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA862260.1756203563109851No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG567190.11552212777587692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG828200.027.686383
AGACCGC826550.027.6817554
GACCGCG832950.027.4582025
ACCGCGT843900.027.1605246
TCAGACC868300.026.9053382
CTCAGAC931900.025.7296831
CCGCGTT907300.025.2650387
CGCGTTC923050.024.8161688
GCGTTCT977750.023.4203939
GAACGAT968600.023.11696245
CGTTCTC1072350.021.7466710
AGAACGA1084950.020.6916944
AATACGG1137550.019.96366732
TACGGAG1135000.019.9088134
ATACGGA1141150.019.90249633
GTTCTCT1182250.019.8393211
CCTCTCA1167650.019.77208519
CAATACG1157000.019.69418531
ACTCCCC1178400.019.60712825
AAGAACG1146000.019.5894843
Produced by FastQC (version 0.11.5)