FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49097953
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA984890.20059695767764493No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG851750.1734797375361046No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT822390.16749985483101507No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG692350.1410140255745489No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG643560.1310767477413977No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC575250.11716374407706977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT340350.014.0452193
GTCACTC326350.013.9787748
GGTCACT330600.013.8126087
AGGTCAC347550.013.4109996
GGAGGTC357600.013.2911124
AGCTTAG273100.012.94017245
ACTCTCC355950.012.84361211
GAGGTCA376900.012.6693425
GTTGTCG31350.012.55896626
ATAGGGG286950.012.32565138
AGGGATC196750.012.2340412
GGCATAG286650.012.22748135
TCACTCT390850.011.8852279
CACTCTC394000.011.59712210
TGGCATA306600.011.49092134
GAGCTTA318750.011.09411844
TGGGAGG347300.010.9634122
TGTCGTT35950.010.7016928
CATAGGG333700.010.56381937
TATCCTA94100.010.4312525
Produced by FastQC (version 0.11.5)