FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23454858
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1560620.6653717536895768No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA522070.22258501842134368No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG275190.11732750631020662No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCC268430.11444537417365733No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT257760.1098962099877134No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTC238310.10160368483151762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG238200.032.054283
AGACCGC238750.031.9521684
GACCGCG246850.030.7652995
TCAGACC257450.030.2604562
ACCGCGT255100.029.823256
CTCAGAC277150.029.7116051
CCGCGTT313750.024.2768447
CGCGTTC342000.022.271528
GCGTTCT357750.021.3287439
CGTTCTC390300.019.8381610
CCGTACG52700.017.9289384
CGTACGC53000.017.9113545
CGCGTAA16650.017.4458241
GTTCTCT447000.017.41739311
TTCGGCG53700.017.38749144
TTCGACG8300.017.3457766
TCCGTAC55400.017.136363
TCGGCGC57800.016.2318745
TACGCCA59850.016.199527
GAACGAT469050.016.1024745
Produced by FastQC (version 0.11.5)