FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44119365
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC454300.1029706569892835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC72400.018.67695625
GTCCGTT28300.016.7833021
CTCCGTT58900.015.9368391
CCCCCTT119900.014.9818391
CCCAGAT198500.014.5041231
AGCACTA97000.014.45062224
TTCGACG13900.014.2439916
GACCGCG37750.014.1253475
ACCGCGT38300.014.098616
CCCGTAT29000.014.0495371
CGTACGC29800.014.0431465
CTACACG40150.013.90652145
CTCGGTT80700.013.8911181
AGACCGC38900.013.7084764
TTGCGTC52950.013.68214543
CTCCGAT49200.013.6800441
AGACGCT266600.013.66315641
CCGTACG31450.013.5217084
CCGCAGA276150.013.49268237
AAGCGGC257650.013.3692628
Produced by FastQC (version 0.11.5)