FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28365323
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA366600.12924231463889907No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG305610.10774070861100365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG125050.013.50849745
ATAGGGG131800.013.00749738
GGGAGGT167500.012.7194663
GGCATAG136200.012.28825735
GAGCTTA137250.012.24240444
GGAGGTC178150.011.8578444
GGTCACT168750.011.6806817
TGGCATA145650.011.55226534
GTCACTC169800.011.3952788
CATAGGG157000.010.87667837
ACTCTCC184400.010.78431811
GCATAGG159950.010.6057136
CGTGGCG90600.010.0423781
TGGGAGG181950.010.0012122
CACTCTC194800.09.954082510
CACCGAA206200.09.92666945
TCACTCT203650.09.7321359
TTTCTAT289850.09.6500971
TTCTATA293050.09.4450342
GCACCGA221600.09.29788544
Produced by FastQC (version 0.11.5)