FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36945419
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA59600.015.77420123
ACGCTAC58950.015.75749324
GACCGCG33150.015.2655625
TTCGACG12700.014.876076
CGCTACC61250.014.8727825
ACCGCGT34500.014.60298256
GTCCGTT32300.014.0774031
AGACCGC37750.013.5251144
CCGGCTT98000.012.301862
AAAACGC78450.012.29935421
GCACTAC50000.012.10111825
GTCCGCT38800.011.7190751
GTCGCGA4450.011.6343341
CAGACCG47450.011.6133263
CCGTACG36800.011.61282354
GTCGGTT33400.011.5245331
CGTACGC37700.011.5140935
GTCCGGT27450.011.3984591
GTCCGAT24300.011.3939031
CTCCGAT38600.011.3132691
Produced by FastQC (version 0.11.5)