FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21846311
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1317350.6030079861080436No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA222900.10203095616463576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG246100.028.5374163
AGACCGC245900.028.4415364
GACCGCG247850.028.172375
ACCGCGT253500.027.6243576
TCAGACC266750.027.1635152
CTCAGAC295750.025.671481
CCGCGTT282800.024.714267
CGCGTTC295600.023.6659538
GCGTTCT315650.022.1030869
CGTTCTC354050.020.28407910
GAACGAT360200.018.78242545
GTTCTCT398700.018.17613211
AATACGG389900.017.68744332
ATACGGA392300.017.54466233
TACGGAG389950.017.51167334
AGAACGA390400.017.38158644
CCTCTCA414000.017.29313519
ACTCCCC412300.017.22836725
CAATACG405250.017.17313831
TCACTCC420850.017.08135623
Produced by FastQC (version 0.11.5)