FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21846311
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA281500.12885470686561223No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT277110.1268452142789691No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG250470.11465093580330335No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG228480.10458516314264683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG14950.012.64220226
TATCCTA44650.011.4401015
AGGGATC69100.011.4324462
CGTCGAT17500.011.18807712
ATATAGC17900.010.9323756
AGCTTAG69800.010.92517545
TGTCGTT16850.010.81577928
ACCCGGC38500.010.6374165
GTCACTC109100.010.4750948
TCACCCG43750.010.3400613
GGCATAG72700.010.30423735
ATAGCTC35250.010.27692839
GGTCACT107750.010.2709367
TAGGCTA46500.010.25832710
GTTAGTT30150.010.2277291
ATAGGGG72850.010.19199238
TCAGACG39350.010.18088152
GCGTCGA19700.010.16595711
GGGAGGT122700.010.1067653
ACTCTCC111900.010.07469911
Produced by FastQC (version 0.11.5)