FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43407462
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2133910.4915998083463161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG23350.011.84981526
GGCATAG112000.010.40384935
ATAGGGG115450.010.1905538
AGCTTAG111600.010.03557245
CATAGGG127450.09.0896737
TGGCATA129750.09.015511534
ACCCGGC65700.08.9777195
AAGCCGG263650.08.92238345
ATAGCTC60550.08.84179739
GCATAGG130550.08.82208636
GAGCTTA131650.08.69569244
GTCACTC167500.08.6936988
GGTCACT167650.08.5653147
TTCTATA276000.08.5185712
AACTCTA83200.08.514645
ATTTCTA240100.08.413482
ACTCTCC174050.08.36543311
CTATAGT270850.08.3291614
TATAGTG285300.08.3170425
GCGTGAA290700.08.28703240
Produced by FastQC (version 0.11.5)