FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17997767
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG9150.025.819076
GACCGCG42600.022.4466615
ACCGCGT43600.021.828626
AGACCGC45400.021.5089074
CAGACCG50300.019.9951763
CGGTATA16250.019.52376220
CCGCGTT51800.018.3295887
CGCGTTC55050.017.2476048
TCAGACC66650.016.8460062
TCGACGA14200.016.6368457
GTTCGAC14600.016.0270255
ACGCTAC62050.014.83084924
CGACGAG14750.014.7963088
AACGCTA62400.014.7837623
CTCAGAC95450.014.4332831
CGCTACC65400.014.07096925
CCCGTAT10100.013.5955911
GCGTACG2501.4879333E-913.50009923
CCGTACG23850.013.3019464
CGTACGC23550.013.2799455
Produced by FastQC (version 0.11.5)