FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17997767
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG47650.011.94838738
AGCTTAG47600.011.76293745
GGCATAG48300.011.46492635
CATAGGG52950.010.66783637
TCAGACG31000.010.5903932
GGGAGGT80850.09.8456453
GAGCTTA58100.09.83056644
CGTGGCG50350.09.7808471
GCATAGG57500.09.70748636
GGAGGTC79600.09.6612654
GTCACTC71300.09.6506748
AGGTCAC78150.09.4089186
GAGGTCA81750.09.2696455
TGGCATA62050.09.25131434
ACTCTCC73900.09.1904711
CGACCCG53450.09.1712756
ACCCGGC20500.09.1042715
TTAACGG24100.09.0505643
AGGGATC41100.09.0274082
TCTATAG98700.09.021923
Produced by FastQC (version 0.11.5)