FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32462212
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26550.024.1356816
GTTCGAC27750.022.6084425
TCGACGA30350.021.113757
CGGTATA38050.021.1055620
GACCGCG91750.019.778665
CGACGAG31950.019.5637978
ACCGCGT94550.019.1431566
AGACCGC96450.019.0713654
CAGACCG102650.018.1166253
CCGCGTT109800.016.5253417
AGTTCGA40200.016.2778744
CGCGTTC114100.015.8434468
ACGAGGA56150.014.41557510
TCAGACC138150.014.389052
CAGTTCG48150.014.2440763
CCCGTAT26100.013.9703791
GTATAGA63650.013.7248522
CTACACG32150.013.63744145
AACGCTA89900.013.600450523
ACGCTAC91900.013.45305324
Produced by FastQC (version 0.11.5)