FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419561
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1962530.4227808186294567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC376750.025.2908084
ACCGCGT383900.024.8311396
CAGACCG388050.024.6882253
GACCGCG387200.024.5670155
TCAGACC422000.023.1772
CCGCGTT414850.022.8697267
CGCGTTC427900.022.251428
GAACGAT455150.020.85236445
CTCAGAC499600.020.5723361
GCGTTCT465250.020.5330459
AGAACGA508550.018.73254644
TTCGGCG64800.018.28553244
CGTTCTC535600.018.2146610
ATACGGA548650.017.37606433
AATACGG552750.017.29600732
TACGGAG549800.017.28246534
TCGGCGC68700.017.18292245
AAGAACG563350.016.8943743
CAATACG573450.016.69911631
ACGGAGA574850.016.58411235
Produced by FastQC (version 0.11.5)