FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419561
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA693100.14931205402825762No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG660830.1423602433465495No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG616860.13288794351157263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187200.017.86016345
ATAGGGG206700.016.15686238
GGCATAG208200.016.06801835
GTTGTCG23600.015.26528226
CATAGGG224150.014.971271537
GAGCTTA229550.014.77081244
TGGCATA233400.014.47856834
GCATAGG237450.014.09814836
GGAGGTC311750.014.0855924
ACCCGGC78950.013.954825
ACTCTCC313000.013.72283111
GGGAGGT337450.013.6458433
GTCACTC329600.013.1595138
GGTCACT334400.012.8155977
CACTCTC351500.012.28298510
ATAGCTC82650.012.00549839
TAGGGGA354500.011.91331239
TCTCCCC363200.011.83473213
TCACCCG94650.011.8301053
ACGGACA90900.011.78489918
Produced by FastQC (version 0.11.5)