FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26549786
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1444890.544219075814773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC235700.029.832284
CAGACCG240200.029.3014933
GACCGCG241500.029.0319585
ACCGCGT245250.028.643096
TCAGACC262350.027.2306922
CCGCGTT277600.025.1753527
CTCAGAC306950.024.6244751
CGCGTTC292050.023.9370278
GCGTTCT319050.021.8820069
GAACGAT333450.020.82232945
AGAACGA356250.019.48977344
AATACGG359400.019.36339432
ATACGGA359950.019.32748433
TACGGAG360600.019.26126534
CGTTCTC368000.019.2281610
CAATACG368150.018.94005831
AAGAACG372200.018.66061843
ACGGAGA372700.018.6236535
CCCAATA381500.018.28321529
CGGAGAG385250.017.9760836
Produced by FastQC (version 0.11.5)