FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26117495
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT873610.3344922627533766No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG490510.1878089763202788No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG400670.15341057785212556No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA294800.11287453103752867No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC290360.11117452113994852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT231050.021.50000645
CAGACCG215250.020.8873563
AGACCGC215350.020.8776154
ACCGCGT221750.020.2138656
GACCGCG236200.019.0156385
CCGCGTT238850.018.7921497
AACGCTA69800.018.79105623
CTCAGAC288350.018.6850191
TCAGACC244950.018.5850492
CGCGTTC242500.018.55488
ACGCTAC71950.018.2295424
AGAACGA280950.017.86553644
GCGTTCT285900.017.752649
AATACGG287900.017.43682732
ATACGGA285300.017.38378333
TACGGAG285000.017.31751634
CAATACG299450.016.9197131
AAAACGC77800.016.91664721
CGCTACC77950.016.79760625
CCCAATA303050.016.66658829
Produced by FastQC (version 0.11.5)