FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24234970
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1266520.5226001930268533No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG600860.24793098567895897No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA267430.11034880587844755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT285300.023.6372031
CGCGTAA8050.023.349261
TTCGACG11500.021.8982646
AGACCGC34600.021.7697224
AACGCTA377800.019.5905223
ACGCTAC377850.019.57067724
CCGGCTT382850.019.304632
CGCTACC382750.019.26224525
AAAACGC389400.019.05772821
GAAAACG391250.018.85075420
AAACGCT395550.018.77855322
CAGACCG42350.018.635363
GACCGCG40500.018.4872555
CGTGGTT399150.018.48083341
TCGTGGT406550.018.07767140
TCACGCT422050.017.8743678
GTTTTCG402900.017.86878445
TTCACGC424050.017.805537
ACGCTCA432050.017.62708710
CGGCTTC434300.017.5765693
Produced by FastQC (version 0.11.5)